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Effects of BEA, ENN B, and MON on intracellular reactive oxygen species (ROS) generation in human Jurkat T cells. Cells were exposed to increasing concentrations of BEA (0–7.24 μM), ENN B (0–17.88 μM), and MON (0–161.80 μM) for 24 h. ROS levels were measured using a Total ROS assay and expressed as relative fluorescence units (RFU). Data represent Mean ± SD of three independent biological experiments (n = 3), with individual experimental values shown as overlayed data points. Asterisks (*) indicate statistically significant differences compared to the control group ( p < 0.05).

Journal: Frontiers in Toxicology

Article Title: Combined exposure to emerging Fusarium mycotoxins produces concentration-dependent synergistic toxicity in Jurkat T cells

doi: 10.3389/ftox.2026.1924250

Figure Lengend Snippet: Effects of BEA, ENN B, and MON on intracellular reactive oxygen species (ROS) generation in human Jurkat T cells. Cells were exposed to increasing concentrations of BEA (0–7.24 μM), ENN B (0–17.88 μM), and MON (0–161.80 μM) for 24 h. ROS levels were measured using a Total ROS assay and expressed as relative fluorescence units (RFU). Data represent Mean ± SD of three independent biological experiments (n = 3), with individual experimental values shown as overlayed data points. Asterisks (*) indicate statistically significant differences compared to the control group ( p < 0.05).

Article Snippet: The Total ROS Assay Kit used for intracellular reactive oxygen species measurement was obtained from Solarbio Life Sciences (catalog number: CA1410; Beijing, China).

Techniques: ROS Assay, Fluorescence, Control

Effects of BEA, ENN B, and MON, individually and in combination, on intracellular reactive oxygen species (ROS) generation in human Jurkat T cells. Cells were exposed to increasing concentrations of BEA, ENN B, and MON, alone or in binary and ternary combinations, for 24 h. ROS levels were measured using a Total ROS assay and expressed as relative fluorescence units (RFU) (A) BEA + ENN B (B) BEA + MON (C) ENN B+ MON, and (D) BEA + ENN B+ MON. Data represent Mean ± SD of three independent biological experiments (n = 3), with individual experimental values shown as overlayed data points. Asterisks (*) indicate statistically significant differences compared to the control group ( p < 0.05).

Journal: Frontiers in Toxicology

Article Title: Combined exposure to emerging Fusarium mycotoxins produces concentration-dependent synergistic toxicity in Jurkat T cells

doi: 10.3389/ftox.2026.1924250

Figure Lengend Snippet: Effects of BEA, ENN B, and MON, individually and in combination, on intracellular reactive oxygen species (ROS) generation in human Jurkat T cells. Cells were exposed to increasing concentrations of BEA, ENN B, and MON, alone or in binary and ternary combinations, for 24 h. ROS levels were measured using a Total ROS assay and expressed as relative fluorescence units (RFU) (A) BEA + ENN B (B) BEA + MON (C) ENN B+ MON, and (D) BEA + ENN B+ MON. Data represent Mean ± SD of three independent biological experiments (n = 3), with individual experimental values shown as overlayed data points. Asterisks (*) indicate statistically significant differences compared to the control group ( p < 0.05).

Article Snippet: The Total ROS Assay Kit used for intracellular reactive oxygen species measurement was obtained from Solarbio Life Sciences (catalog number: CA1410; Beijing, China).

Techniques: ROS Assay, Fluorescence, Control